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Probing Native Protein Conformations with IMgenius™

· Protein Structure,Ion Mobility

This is a new Application note about a very interesting topic

Based on the current industry drive for accelerated drug development, there is aheightened need for screening native protein structures. Atmospheric pressure Ion MobilitySpectrometry (IMS) is an emerging analytical technology that addresses this challenge.IonDX Inc. has developed a bench-top analytical system, IMgenius™, based on thistechnology.

Our patented spectrometer simultaneously measures five important attributes of proteinsand biotherapeutics:• Size distribution• Structural conformation• Stability, including aggregation• Comparability• Purity

Charge-reduced electrospray: Desalted samples are electrosprayed at 0.3 uL/minfollowed by passing the electrospray droplets through a charge-reducing chamber wheredroplet charge is reduced to a single charge. Charge reduction is accomplished by exposing the droplets to bipolar air ions produced when alpha particles are emitted by a safely disposable sealed Polonium source. In this way protein ions never pass through high charge states and remain in their native conformation, allowing native conformations to be studied.

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